The USDA’s Food Safety Inspection Services (FSIS) has declared Salmonella as an adulterant in raw breaded stuffed chicken products that exceed 1 CFU/gram, necessitating the development of methods for quantifying Salmonella in poultry samples.
To date, limited research has been conducted on the development of these methods. Currently, only three relative quantification-based PCR assays are available for quantification of Salmonella levels, which provides a very low number of options. Recent independent validation studies on one of the available methods have shown a lack of reproducibility, further limiting available options for the poultry industry. Meanwhile, another current option for detecting and quantifying Salmonella uses a complex DNA extraction protocol. Read more